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We are seeking a highly motivated and experienced Associate Scientist/Scientist to join our Strain Engineering team focused on applying multiplexed HTP approaches for enzyme and host engineering in microorganisms, particularly yeast.
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GenScript's businesses encompass four major categories based on its leading gene synthesis technology, including operation as a Life Science CRO, enzyme and synthetic biology products, biologics development and manufacturing, and cell therapy.
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Core competencies include enzyme and competent cell manufacture, next gen sequencing services and library preparation kits, to molecular diagnostics, applied, government and academic customers.
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Demonstrated ability with cell-based assays and molecular biology techniques including, but not limited to, immunoassays (ELISA, Western Blots), PCR, qPCR, and flow cytometry. Experience in biochemical assays development, enzymology research methods, and enzyme inhibition studies via techniques including spectroscopy (absorbance, fluorescence, and luminescence), and mass- spectroscopy.
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Work-related experience with molecular biology testing procedures (such as PCR, Sanger Sequencing, Next-Generation Sequencing). Work-related experience with immunoassays, enzyme activity assays, and/or HPLC.
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This role is a growth-based opportunity to acquire extensive, diverse experiences and skills with microbial physiology, strain design, biosynthetic pathway optimization, enzyme development, and fermentation science.
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Solid technical ability and hands-on experience with molecular biology, ribonucleic acid ( RNA) and protein analysis, analytical gel electrophoresis, quantitative polymerase chain reaction (qPCR),enzyme-linked immunosorbent assay (ELISA), western blot, flow cytometry, quantitative reverse transaction (qRT-PCR), microscopy, histology, and cell culture.
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The research assistant will be responsible for performing tissue culture and basic molecular and biochemical lab techniques (PCR, immunoprecipitations, western blots) and will have the opportunity to learn early drug development, proteomics, and genomic screening approaches.
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Apply in-depth molecular biology knowledge and experience in the development of CRISPR-based molecular diagnostic assay systems, including development of enzyme reagents and assay workflows, consumables, and instrumentation.
$150,000 - $190,000 a yearFull-timeExpandApply NowActive JobUpdated Today - UpvoteDownvoteShare Job
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The department of Biology is a large, unified department with strong undergraduate degrees, nationally-ranked graduate programs, and world-class research spanning the breadth of biological questions and experimental systems ¿ from ecosystems to microbiology and developmental biology, from evolution to cell biology, from molecular biology to systems biology, bioinformatics, and genomics.
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Molecular biology experience with experience in enzyme processes and optimization, IVT and/or other isothermal amplification, and PCR. Molecular biology experience with experience in enzyme processes and optimization, IVT and/or other isothermal amplification, and PCR.
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PhD degree and relevant formal academic qualification in Biochemistry, Molecular Biology, Synthetic Biology or related discipline. An enzyme called reverse transcriptase (RT) is the most foundational tool for converting RNA into cDNA, enabling sensitive detection and RNA sequencing.
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Research Scientist | Molecular Biology. Experience with enzyme engineering, including rational design and direction evolution is preferred. Enzyme engineering using rational design and directed evolution.
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Perform gel-electrophoresis (both DNA and protein gels) and Immunoblotting Perform small molecule/ligand binding assays and/or enzyme activity assays Assist protein structural analysis (such as protein/peptide NMR analysis) Assist proteomic analysis, including sample harvesting May participate in basic molecular cloning (plasmid construction and analyses) and PCR-based genotyping May participate in plant care.
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Experience with RNA and protein extraction and digital droplet PCR (ddPCR) is highly valuable with other molecular biology techniques including Jess Simple Western and plate-based assays is a plus.
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