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Some experience with some basic molecular biology techniques is a plus (e.g. ELISA, Western Blots, protein assays, cloning, RNA and DNA isolation). Dr. Wolf’s laboratory is looking for a highly motivated Research Associate II interested in the innate inflammatory responses of myeloid cells to join the Department of Biomedical Sciences and the Inflammatory Bowel Disease Institute (IBDI) at Cedars-Sinai.
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She/he will perform advance cellular and molecular biology techniques under the supervision of the lab manager and PI. She/he will work independently performing several techniques including cell culture, cell transfection, lentiviral particles preparation, flow cytometry, ELISA, molecular biology techniques and animal models.
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The candidate will have experience working with mice, stereotaxic techniques, small animal surgeries, cell culture, and molecular biology. The successful candidate will conduct multidisciplinary in vitro and in vivo research with a variety of approaches including biochemical assays, western blotting, immunocytochemistry, RT-PCR, Elisa, confocal microscopy, and neural cell culture.
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Perform routine procedures in bacterial culture; molecular biology (e.g., DNA and RNA purification, PCR); clone, produce and analyze recombinant polypeptides; and immunological assays (e.g., ELISA); work with live animals (mice.
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The Yoh candidate will be expected to apply expertise across several practical skills, including immuno-serology, cell-based assays, molecular biology, and primary cell culture. Experience with molecular biology including but not limited to molecular cloning, protein characterization, etc.
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Perform cell biology and molecular biology including primary and tumor cell culture, lentiviral transductions, RNA and protein preparation from cells and tissue, RT-PCR, western blotting, and ELISA. Some training may be provided.
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Basic knowledge and skills in protein biochemistry and molecular biology are required. protein characterization, western blot, pcr, elisa, Biochemistry. protein characterization, western blot, pcr, elisa.
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Strong technical and scientific background in current molecular biology methods including RT-PCR/PCR, and DNA/RNA sequencing platforms. PhD and/or MSc in Molecular Biology or related field.
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PhD with 2-5 years relevant industry experience, MS with 5+ years’ industry experience, or BS with 7+ years’ industry experience, with degrees in Biochemistry, Molecular and Cellular Biology, Chemical Engineering, Bioengineering, Chemistry or related field.
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Hands-on experience with basic molecular biology techniques (including but not limited to RNA/DNA isolation, PCR, RT-PCR, SDS-PAGE, Western Blot, ELISA, Microscopy) The ideal applicant should possess direct experience in various molecular techniques, a robust understanding of cell biology, and excellent organizational and presentation skills.
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At least 4 years of experience related to molecular biology, cellular biology, experimental design, and assay development. g, biochemistry, cell biology, molecular and analytical chemistry) and different assay platforms is expected to be part of this work.
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Ph. D. in cell/molecular biology, pharmacology, neuroscience, or related field with 2+ years of relevant experience. This individual should have demonstrated experience in molecular mechanisms of neurodegeneration and will be responsible for leading early drug discovery efforts to validate novel drug targets in this area.
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Skillsets of interest include expertise in tissue culture, biochemical tools (SDS-PAGE, immunoblotting, ELISA, protein assay), and a good working knowledge of molecular techniques including generation of mutant plasmids, transfection with plasmids/siRNA for transient protein expression in cell-based reporter systems as well as use of CRISPR and transcriptomics/proteomics technologies.
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Primary responsibilities may include buffer preparation, drug formulation, endotoxin testing, animal tissue processing, DNA/RNA/protein isolation, spectrophotometry, chromatography, immunochemistry, microscopy, image analysis, enzyme activity assays, protein/oligo/small molecule interaction techniques (ELISA, SPR, BLI, MST, FRET, etc), various molecular biology techniques (PCR, qPCR, ddPCR, dPCR, SDS-PAGE, etc.
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Experience in genetic manipulation of cells via CRISPR and viral transduction, running cellular (2D and 3D viability, apoptotic, cell cycle), molecular and biochemical (q-PCR, western blot, ELISA, Jess) assays.
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