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We employ techniques such as DNA sequencing for genome analysis, RNseq for differential expression analysis, ATAC-seq for cis-regulation, single-cell RNseq for cell identification and trajectories, and in situ hybridization and immunohistochemistry for visual validations.
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Laboratory experience with bacterial cultures and real-time PCR and/or library preparation for next-generation DNA sequencing. Assist with laboratory research according to protocols, including sample processing, culturing bacteria, biomolecule isolation, and molecular analyses including PCR, real-time PCR, and whole genome and amplicon sequencing library preparation.
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Molecular biology and biochemistry: RNA and DNA extractions, cloning, mutagenesis, quantitative PCR, protein immunoprecipitations, Western blotting, and proteomic sample preparation. Next generation sequencing workflows: library preparation, RNAseq, ATAC-seq, ChIP-seq, Cut-and-Run.
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Volta Labs is an MIT spin-off that has unlocked powerful new DNA sequencing capabilities by developing novel fluidic technology. Building off of this core technology, Volta has created a product that streamlines DNA sequencing sample prep.
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The ideal candidate will have a strong track record of commercial success in the RNA/DNA sequencing space, with experience selling enzymes and reagents into academic, commercial and biopharma labs and research facilities.
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We also use bioinformatics pipelines and develop novel bioinformatics approaches to analyze data types such as single cell RNA sequencing data, spatial transcriptomics data, epigenetics data such as DNA methylation and ATAC sequencing data, and others.
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Demonstrated experience with gene synthesis, cloning, and DNA sequencing techniques. Experienced Senior Process Development Associate will be responsible for independently executing bench-scale experiments to develop, characterize, and optimize manufacturing processes involving production of protein biologics and plasmid DNA. The Senior Associate will work with scientists, engineers, operators, and other associated groups to optimize, implement, and tech transfer new processes and to troubleshoot any process-related problems.
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At Centrillion , we are at the forefront of multiple cutting-edge fields in genetics and biotechnology, including spatial genomics, ultra long DNA sequencing, rapid on-chip sequencing, viral sequencing, genotyping, and synthetic biology.
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Molecular biology techniques such as DNA/RNA purification, PCR, cloning, and western blotting will be used on a daily basis. This individual should also know how to implement and troubleshoot PCR protocols including Single Genome Amplification, next generation sequencing library preparation, sequencing chemistries, and high throughput purification methods.
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The Gene Manufacturing Supervisor will be very knowledgeable of basic synthetic/molecular biology skills (PCR, vector production, cloning, miniprep, DNA analysis, sequencing, packaging) and will perform, train and/or oversee the production staff on basic molecular biology workflows in a highly automated, high-throughput manner.
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DNA/RNA sequencing platforms (e.g., next-generation sequencing [NGS] and/or single molecule real-time sequencing [SMRT]), and bioinformatic skill. Strong technical and scientific background in current molecular biology methods including RT-PCR/PCR, and DNA/RNA sequencing platforms.
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Experience with basic molecular biology techniques, including but not limited DNA and RNA extraction, PCR, ligation, DNA sequencing. The primary duties will be associated with DNA sequencing services (Capillary, Illumina, and PacBio Sequel instruments) as well as general lab duties.
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DNA sequencing library prep. Next-generation sequencing data analysis. Work with biologists on projects related to biochemistry and molecular biology, system and signal engineering, virology and microbiology, marine biology, and computational biology.
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TCR repertoire analysis from DNA and RNA sequencing data. 4+ years of experience in computational biology for immuno-oncology applications (academic experience during PhD is counted). We are currently looking for a computational cancer biologist in the field of immuno-oncology to join our interdisciplinary team.
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Proficiency with the following techniques required: DNA sequencing, gel electrophoresis, molecular biology techniques, PCR, DNA sequence analysis tools. Experiments will involve modern molecular biology techniques, next generation DNA/RNA sequencing, primary sample processing and data analysis.
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