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Covaris has developed the Adaptive Focused Acoustics™ (AFA) technology platform, which enables pre-analytical sample preparation, enabling the extraction, purification, and collection of nucleic acids, proteins, and metabolites from a wide variety of samples.
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Knowledge and hands-on experience with molecular biology, especially PCR, DNA purification and analysis; Knowledge and hands-on experience with molecular biology, especially PCR, DNA purification and analysis.
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Testing performed: HLA Flow PRA screen; Luminex Single Antigen (LSA) HLA Class I & II; Flow cross-matching; ELISA; cell isolation and preservation from peripheral blood and donor tissue specimen; DNA extraction, purification, and quantitation; various molecular typing techniques to include Next-generation sequencing (NGS), RT-PCR, and low to intermediate resolution SSO/SSP typing.
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2+ years of experience with standard mRNA workflow: e.g. plasmid/DNA linearization, purification, RNA synthesis by IVT, and purification. The ideal candidate will plan and execute production and purification of novel RNA molecules, while demonstrating agility to learn new tools and techniques to characterize purity of the RNA molecules.
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Experience in various methods of nucleic acids purification (DNA, RNA) 3. Minimum of 2 years of biochemistry, analytical chemistry (quantitative analysis) or molecular biology laboratory experience.
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Support general lab activities such as: making solutions and buffers as needed and performing basic molecular cloning (primer design, PCR, DNA/RNA purification), as well as performing protein expression and protein purification and other molecular biology techniques.
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1 year in molecular biology techniques with hands on expertise in DNA/RNA extraction, PCR, q-PCR, mutagenesis & assembly reactions, SDS-gel page, electrophoresis, cloning replication - pDNA techniques, western, northern, southern blotting, different cell line cultures, affinity chromatography, ELISA, microfluid liquid handling system, and thin layer chromatography.
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Knowledge of molecular biology techniques, including DNA construct design and cloning, DNA purification, immunoprecipitation. The Susa lab is starting the Department of Pharmaceutical Chemistry at UCSF and is seeking applicants for a research technician position using structural biology, membrane protein biochemistry, cell biology, and protein engineering to understand fundamental mechanisms of transmembrane signaling within the human immune system.
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Additionally, perform recombinant DNA techniques to include preparing plasmids and maintaining plasmid stocks, analyzing DNA and RNA purification, and conducting DNA electrophoresis, and RT-PCR. Make innovative contributions regarding experimental design and methodology, using published scientific data in referred journals, as well as online using search engines and subject-specific websites, to make recommended changes to current protocols and practices.
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Under general supervision performs complex laboratory techniques including, but not limited to recombinant protein purification, DNA purification, flow cytometry. We use high resolution electron cryo-microscopy (cryo-EM) and image processing to explore the molecular design of large, multicomponent supramolecular assemblies.
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The candidate will be expected to generate mutant strains and conduct microbiology and molecular biology techniques, including fungal and macrophage manipulation, cloning, protein purification, and biochemical assays.
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Basic laboratory experiance with DNA extraction and purification from plant samples and Polymerase chain reaction (PCR) technology. Prior experience working with Loboratory Information Management Syetems (LIMS), knowledge of data analysis, optimization in DNA extraction, worked in/experience with different protocols and tissue types.
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Experience with Cytiva platform using Unicorn software (revision 7.0 preferred) for purification of PCR-produced DNA product highly desired. Our client is seeking a pro-active and methodical scientist, dedicated to providing exceptional post-processing product to GMP quality standards and customer requirements, as well as routine and investigative analysis of DNA product.
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Mammalian cell culture, purification of RNA and DNA. Prepare bacterial growth media and carry out protein purification in bacteria. Basic knowledge & skills, including PCR. Western blotting, SDS-PAGE gels, expression and purification of His-tagged proteins, Mammalian cell culture.
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Perform or assist in routine procedures in bacterial culture; molecular biology (e.g., DNA and RNA purification, PCR); clone and produce recombinant polypeptides; and immunological assays (e.g., ELISA.
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