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Experience with molecular biology techniques, such as PCR, DNA purification and quantification methods. Sequence various types of libraries using the Illumina and Oxford Nanopore NGS platforms.
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Hands-on experience with molecular biology techniques like PCR, DNA cloning using in-fusion/Gibson assembly/Golden Gate, plasmid/primer design, DNA purification, Sanger Sequencing analysis, and NGS library preparation for Illumina platforms is preferred.
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Preferred with at least 1 year molecular lab experience including DNA/RNA extraction, library preparation and NGS analysis. The NGS laboratory is a new initiative of the Clinical Pathology Department at Beth Israel Deaconess Medical Center to provide oncology laboratory diagnostic services using cutting edge technology.
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Daily use of traditional molecular biology processes and techniques especially PCR, DNA quantitation, handling, and purification. Hands-on experience with NGS and NGS applications (e.g. Running sequencers, performing whole genome sequencing, target enrichment, single cell sequencing, RNseq, exome-seq, ChIP Seq, or similar.
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Functional characterization (in vitro DNA cleavage) of differential CRISPR-Cas RNP complexes by electrophoretic mobility shift assay (EMSA) or anion exchange (AEX) chromatography. Experience in NGS based assay development and implementation.
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Prepare the Next Generation Sequencing (NGS) library. Familiarity of and experience working with DNA manipulation techniques, enzymatic reactions, and sequencing chemistry. Operate quantification, sizing, and NGS instruments.
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NGS, RNA and DNA analysis. Experience with Cell culture methods (T cells, CAR-T or TCR-T preferred), CRISPR based gene knockout, quantitative PCR and functional characterization of knockout T cells (Cytotoxicity assay using Incucyte or xCelligence, and MLR assay.
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Working knowledge of Next-Generation Sequencing (NGS), tissue culture, and molecular biology is preferred. Performing high throughput molecular biology techniques including PCR, cloning, DNA isolation and Next Gen sequencing.
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Similar to Next Generation DNA Sequencing (NGS), which gave us a blueprint of what could happen by studying our DNA, NGPS enables an unbiased view of the proteome through improved scale, resolution, and sensitivity leading to better understanding of disease and improved general health.
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Hands-on experience with NGS and DNA/RNA molecular biology is required. In this role, you will work as part of the QC team and responsible for effectively managing the QC process of Takara’s Reproductive Health Technology (RHT) and Next Generation Sequencing (NGS) product portfolio.
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Testing performed: HLA Flow PRA screen; Luminex Single Antigen (LSA) HLA Class I & II; Flow cross-matching; ELISA; cell isolation and preservation from peripheral blood and donor tissue specimen; DNA extraction, purification, and quantitation; various molecular typing techniques to include Next-generation sequencing (NGS), RT-PCR, and low to intermediate resolution SSO/SSP typing.
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The Staff Scientist, Bioinformatics is responsible for collecting product requirements and designing and developing NGS analysis pipelines to meet those requirements. As a Staff Scientist, Bioinformatics, you will be part of a growing team of bioinformaticians primarily focused on single-cell and immune profiling NGS applications.
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NGS library design, preparation, QC, and pipeline construction for NGS. Biophysical studies of DNA target-binding affinity of CRISPR-CasX effector complexes using BLI. sgRNA sequencing for identity confirmation by NGS.
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Analysis may include immunological procedures including Enzyme Linked immunoassay (ELISA), Western blot, Flow Cytometry and RNA/DNA sequencing assays. Perform research in next generation sequencing (NGS) including Illumina (iSeq, MiniSeq, MiSeq, NextSeq 2000 and NovaSeq 6000), and Oxford Nanopore Technologies (MinION and GridION.
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3+ years hands-on experience with DNA sequencing such as raw sample preparation, nucleic acid extraction, library preparation, and NGS sequencing. (Candidates with hands-on experience with DNA sequencing such as raw sample preparation, nucleic acid extraction, library preparation, and NGS sequencing are encouraged to apply and we can level the position to the experience.
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