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Functional characterization (in vitro DNA cleavage) of differential CRISPR-Cas RNP complexes by electrophoretic mobility shift assay (EMSA) or anion exchange (AEX) chromatography. Founded by CRISPR inventors and leading molecular engineers Benjamin Oakes, Brett Staahl, David Savage, and Jennifer Doudna, Scribe is overcoming the limitations of current genome editing technologies by developing custom engineered enzymes and delivery modalities as part of a proprietary, evergreen CRISPR by Design platform for CRISPR-based genetic medicine.
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This role is an excellent opportunity to learn a number of valuable, cutting-edge techniques in CRISPR-based knock-in (CRISPR-ki) genomics, cell biology, and proteomics while working with a dynamic and interdisciplinary research team.
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Design and execution of experiments to identify best-in-class mRNA design for in vivo delivery of CRISPR gene-editing systems. We are seeking a highly creative, passionate, and motivated individual to join us in our quest to develop the next generation of CRISPR-based therapeutics.
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You will routinely design and perform genome editing using CRISPR tools and other molecular biology techniques such as PCR, Gibson assembly, DNA transformation, and DNA sequencing analysis. Proficient and thoughtful in designing and performing CRISPR-based genomic editing.
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To accelerate and expand its efforts,CRISPR Therapeuticshas established strategic partnerships with leading companies includingBayerand Vertex Pharmaceuticals. Since its inception over a decade ago,CRISPR Therapeuticshas transformed from a research-stage company advancing programs in the field of gene editing, to a company with a diverse portfolio of product candidates across a broad range of disease areas including hemoglobinopathies, oncology, regenerative medicine, cardiovascular and rare diseases.
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Mammoth Biosciences is a biotechnology company focused on leveraging its proprietary ultracompact CRISPR systems to develop long-term curative therapies, as well as other applications such as decentralized precision diagnostics.
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Responsible for transfection, lentiviral transduction, flow cytometry, cell sorting, next generation sequencing, & performing CRISPR screens in neuronal & glial cell types. Perform pooled & arrayed CRISPR screens with FACS-based molecular & functional assays on immortalized cell lines, primary cells, & iPSC-derived cells.
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Develop and maintain data processing pipelines for CRISPR screen analysis, sgRNA library design, and data integration from CRISPR screens with other data types. Specifically, the candidate will work on developing and applying cutting edge approaches for modeling massive single cell and bulk multi-omic datasets and CRISPR screen data.
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Recent work from the group includes in vivo and RNtargeting CRISPR screens, pairing CRISPR perturbations with multi omic single-cell readouts, engineering T cell therapies and precise genome editors, and uncovering regulatory elements in the human noncoding genome.
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Corteva Agriscience has an opening for an experienced patent agent at its facility located in Johnston, Iowa. This is a highly visible, key role within Corteva's Intellectual Property group which provides an exciting opportunity to work closely with an in-house legal team, brilliant scientists and business partners involved in a variety of world-class technologies such as advanced breeding, genomics, CRISPR and artificial intelligence.
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Registered to Practice before the USPTO as a Patent Agent. Minimum 5-7 years of experience in drafting and/or prosecuting patent applications as a Registered U.S. Patent Agent, preferably in a law firm.
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Experience with gene knockdown techniques such siRNA, shRNA or CRISPR. Experience with multi-color flow cytometry and high throughput assays such as CTG, HiBiT, or live cell imaging techniques such as Incucyte.
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Have strong, in-depth molecular biology laboratory experience in designing and executing complex molecular screens, especially MPRA and large CRISPR screens. candidate will be proactive, well-organized, and technically capable of designing, optimizing, and executing complex molecular screens including Massively Parallel Reporter Assays (MPRAs) and large (50,000+ sgRNA) CRISPR screens.
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This leader and the team will need to coordinate cross-functionally with Process and Analytical Development, Internal and External Manufacturing, Validation and Metrology, Quality Control and Analytical Technologies (QCAT), Quality Assurance, and Regulatory to support process validation and manufacturing of gene editing components and ex vivo CRISPR gene-edited cell therapies both internally and at Contract Manufacturing Organizations (CMOs.
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Experience with analyzing single-cell RNA sequencing, proteomics or pooled CRISPR screening datasets. Experience with analyzing single-cell RNA sequencing, proteomics or pooled CRISPR screening datasets.
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