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Flow cytometry, fluorescent microscopy, suspension cell culture, lentivirus generation, SDS-PAGE and western blot, viral titering by TCID50, cell-based assays, ELISA, affinity chromatography, complement assays, Prism software.
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Proven experience in laboratory cellular biology techniques applied to development of physiologically relevant cellular assays to evaluate biological mechanisms (including but not restricted to primary cell culture, flow cytometry and ELISAs.
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The Associate Scientist II is responsible for performing assigned experimental tasks to support the development and optimization of cell line development and cell culture processes for Alexion’s therapeutic protein candidates.
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Experience with standard cell and molecular biology experimental design and techniques such as cell culture, lentiviral production, stable cell line generation, western blot, and qPCR.
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Stemson Therapeutics Corp, a pioneering cell therapy startup, is seeking a Senior Research Associate/Associate Scientist to join our dynamic research and development team. - Strong expertise in mammalian cell culture, across a variety of plate and flask formats.
$75,000 - $103,000 a yearFull-timeExpandApply NowActive JobUpdated Yesterday - UpvoteDownvoteShare Job
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As part of the MSAT group, you will have opportunities to work on and specialize in expertise of Upstream (US) Cell Culture and/or Downstream (DS) Purification. Bionova is a rapidly growing, biologics CDMO focused on developing and manufacturing recombinant protein products using mammalian cell culture processes.
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Experience with Cell culture methods (T cells, CAR-T or TCR-T preferred), CRISPR based gene knockout, quantitative PCR and functional characterization of knockout T cells (Cytotoxicity assay using Incucyte or xCelligence, and MLR assay.
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We are seeking a Principal or Distinguished Scientist specializing in cotton fiber development to guide our efforts in isolating, cultivating, and optimizing stable plant stem cell lines for fiber production.
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Key words: mRNA, DNA, Cell Culture, Bioassay, Molecular Biology, Analytical Development, Process Development, Nucleic Acid Purification, LNP. Plan and execute studies requiring molecular biology techniques including E. coli cell culture, plasmid preparation, RNA synthesis, and purification with the aim to develop the processes that can be scaled up and transferred to MSAT labs.
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Working knowledge of Next-Generation Sequencing (NGS), tissue culture, and molecular biology is preferred. The successful candidate will have experience with general molecular biology, cell biology techniques and be familiar with automation.
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Cell and virus culture, Molecular biology, qPCR, RT-PCR, ELISA, and ELISPOT. Viral analytics: virus rescue and amplification, plaque assay, TCID50, IFA, Cell-based assays: Cell proliferation and cytotoxicity assay, cell-signaling pathways, Molecular cloning of viral vectors for gene therapy or oncolytics.
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Proven expertise in cell culture techniques and a high level of proficiency in molecular biology techniques, including ELISA and PCR. Lead the cultivation and expansion of mammalian cells, overseeing the maintenance of in vitro cell culture studies.
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Independently conduct 3D cell culture, total RNA extraction and measurement, quantitative reverse transcriptase polymerase chain reaction (qRTPCR). The researcher will work as a team with a postdoctoral scientist on a highly collaborative and translational project to optimize and validate a novel prognostic biomarker of wound healing using lab-prepared samples and clinical samples collected from patients with chronic diabetic foot ulcers as part of an ongoing national clinical trial.
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Experience in aseptic cell culture and cell-based assay development. Job Details: Senior Scientist, Bioanalytical. Senior Scientist, Bioanalytical. Design and build custom LBA and cell-based pharmacokinetic, immunogenicity and biomarker assays suitable for detection of target analytes in biological matrices.
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Oversee laboratory hygiene and cell culture protocols, and conducts education of new trainees to ensure these protocols. Creates and conducts experiments related to molecular and cell biology, and analyzes results.
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