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Experience in areas of rodent models (e.g., neural behavioral assays, tissue sectioning/imaging), molecular biology (e.g., PCR, qPCR), and cell biology (e.g., general cell culture, viral preparation), would be a plus.
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Cell biology techniques: Mammalian cell culture, in vitro cellular assays (cell viability assay, proliferation, invasion, apoptosis), luciferase assay. Molecular biology techniques: DNA/RNA extraction, gene expression analysis (PCR/RT-PCR), western blotting, ELISA, Next Generation Sequencing, flow cytometry.
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Experience with Cell culture methods (T cells, CAR-T or TCR-T preferred), CRISPR based gene knockout, quantitative PCR and functional characterization of knockout T cells (Cytotoxicity assay using Incucyte or xCelligence, and MLR assay.
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Extensive experience with mammalian cell culture and aseptic technique required. Health & Wellness: Comprehensive medical coverage and company paid Life Insurance, Disability Insurance & AD&D Work life balance with Paid Holidays and PTO Fitness and cell phone subsidies, and additional benefits such as Aflac, legal services, and pet insurance Employee Assistance Program offering around-the-clock counseling.
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Knowledge of cell culture; Western blot; PCR, fluorescence microscopy; histology; and mouse surgery. Knowledge of cell culture; Western blot; PCR, fluorescence microscopy; histology; and mouse surgery.
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BS/MS in Biochemical Engineering, Biology, Biotechnology or related and 1-2+ yrs of experience with cell culture, ELISA, PCR, cell based assay or flow cytometry. In this role you will provide daily help in cell culture, analytical testing and sample management in support of fast paced commercial manufacturing of cell lines used as a method reference in testing of raw material, final drug product and stability testing of Cell Therapy products.
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Perform research and experiments including tissue sectioning, immunohistochemistry, western blotting, and/or cell culture. This agenda covers a broad range of specialized disciplines, including biochemistry, biophysics, molecular biology, structural biology, genetics, genomics, bioinformatics, cell biology, developmental biology, tumor biology, microbiology, immunology, pathogenesis and neurobiology.
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Perform cell biology and molecular biology including primary and tumor cell culture, lentiviral transductions, RNA and protein preparation from cells and tissue, RT-PCR, western blotting, and ELISA. Some training may be provided.
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Performs testing in support of product manufacturing, employing manual and automated analytical equipment for a variety of molecular, protein and cell based assays, such as PCR, ELISA, and flow cytometry.
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Under initial supervision and training of the faculty investigator, follows established procedures or protocols and utilizes aseptic technique to perform experiments which may include: iPS culture and differentiation, mammalian tissue culture, PCR, gel electrophoresis, Western blotting, laboratory reagent preparation, and other basic molecular biology techniques.
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Preferred qualifications: experience with mammalian cell culture, molecular biological techniques such as electrophoresis (DNA gel and SDS PAGE), Western, and PCR. Able to prepare and maintain laboratory stock reagents and supplies and maintain excellent organizational skills.
$16.55 - $23.28 an hourFull-timeExpandApply NowActive JobUpdated Yesterday - UpvoteDownvoteShare Job
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Ability to do genotyping/sequencing for mouse colony development and cell culture work and maintenance. Perform genotyping for mouse colony and cell culture work and maintenance of cell lines.
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Request biochemistry and molecular biology skills: Western blotting, ELISA, ICC, IF, RNA/DNA, PCR, RT-PCR, Chip, Luciferase report assay, and/or cell culture of neuron, astrocyte, microglia, and human iPSC, etc.
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Must have experience with diagnostic, clinical and immunological including but not limited to ELISA, multiplex immune assays such as Luminex, Quanterix or MSD, qRT-PCR, cell culture, piccolo tests, etc.
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Basic biomedical wet lab skills are necessary (e.g. PAGE, western blotting, PCR, site directed mutagenesis, protein expression and purification); Cell culture, general biochemistry, and cell biology knowledge is important.
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