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Provide assistance in the execution of experiments and analytical procedures including: molecular biology, DNA cloning, cell culture, protein production, ELISA, Biolayer interferometry and HPLC.
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Hands-on experience in next-generation sequencing, e.g. Illumina, Oxford Nanopore or PacBio platforms with various sample types, including purified DNA, microbial, fresh and FFPE tissue material, blood, etc.
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Perform gel-electrophoresis (both DNA and protein gels) and Immunoblotting Perform small molecule/ligand binding assays and/or enzyme activity assays Assist protein structural analysis (such as protein/peptide NMR analysis) Assist proteomic analysis, including sample harvesting May participate in basic molecular cloning (plasmid construction and analyses) and PCR-based genotyping May participate in plant care.
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Our work is highly interdisciplinary and touches on aspects from many fields including bioengineering, protein engineering, biochemistry, synthetic biology, molecular biology, microbiology, structural biology, genetics, bioinformatics, and quantitative biology.
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The incumbent must have experience and interest in the collection and identification of insects, and the isolation of microbes and DNA. Knowledge of current methods used in microbiology and bioinformatics, including techniques used in molecular biology, genetics, and biochemistry is highly desirable.
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Basic skill sets required: NGS (library prep, running Illumina Sequencers), Primary T Cell culture, Crispr KO, Transduction, ELISA, Flow Cytometry, DNA/RNA extraction, and PCR. Bachelor’s degree in Molecular Biology, Biology, Biochemistry, Biomedical Engineering or equivalent.
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Conducting timed pregnancy (occasional), DNA/RNA purification, and PCR-based genotyping while maintaining well organized and rigorous records. Duties include breeding colonies, DNA/RNA purification, and PCR-based genotyping while maintaining well-organized and rigorous records.
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Duties will include basic maintenance of mammalian cell cultures such as changing media, passaging cells and making stocks, molecular analysis including extraction, amplification and sequencing of genomic DNA and RNA, as well as PCR, RT-qPCR, immunostaining, and imaging using confocal microscopy.
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Under supervision, the incumbent will independently participate in planning and executing various laboratory activities to study gene regulation in the mammalian system that requires a combination of knowledge and skills in multiple scientific disciplines related to next generation sequencing technologies, genomics, molecular biology, cellular biology, and biochemistry.
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Minimum of 2 years of biochemistry, analytical chemistry (quantitative analysis) or molecular biology laboratory experience. Experience in various methods of nucleic acids purification (DNA, RNA) 3.
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The selected candidate will participate in a team working with mouse and human beta cell lines and diabetic mouse models, performing DNA/RNA/protein purifications, advanced molecular biology techniques, and developing new molecular biology protocols.
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Bachelor's Degree in Biology, Molecular Biology, Biochemistry, Virology, or cognate discipline. Experience in DNA cloning techniques, including restriction digest, Gibson assembly, PCR, ligation and bacterial transformation.
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Intellectual Property - Life Sciences - Midlevel/Senior Associate Attorney – Boston OfficeRequirements:Boston, Massachusetts office of an AMLaw 100 firm is seeking a midlevel to senior associate with 1-7 years of patent prosecution experience in a biotechnology practice and an advanced degree, preferably a Ph. D., in a life sciences technology area, e.g. molecular biology, immunology, biochemistry, cell biology, and the like.
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Ideally, this person should have experience with molecular biology (plasmid design and generation, DNA/RNA prep, DNA detection, PCR/qPCR etc), heterologous protein expression, protein biochemistry (protein detection, protein characterization and protein purification etc.
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Experience with current molecular biology techniques including in vitro transcription and translation, DNA/RNA extraction, PCR, and NGS. We are looking for an enthusiastic, independent, and self-motivated bench Scientist with a background in mammalian cell culture, RNA biology/Biochemistry and genome editing to support efforts developing curative gene editing therapies and gene edited cell therapies.
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