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The Sanders-Brown Center on Aging is seeking a senior, experienced scientist to lead the research projects extramurally-funded in the Wang Lab. This position requires strong experience in biochemistry & molecular biology, cell culture and transfection, and microRNA biology.
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We are looking for an enthusiastic, competent, and self-motivated Principal Associate Scientist with a background in mammalian cell culture and genome editing to help expand our discovery efforts.
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Experience with mammalian cell culture, aseptic technique, and ELISA binding assays/other plate-based assays is preferred. BS and 1-5 years' experience or MS with 0-3 years' experience with degree in Biochemistry, Molecular and Cellular Biology, Chemical Engineering, Bioengineering, Chemistry or related field.
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Cell Culture, Lab Maintenance, and Lab Support Functions Quality Systems and Documentation Support. The QC Associate will participate in sample preparation teams and support operations such as cell culture, lab equipment preventive maintenance, buffer preparation, supply inventory management, and general support tasks in order to allow the lab to continuously operate.
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The specialist will perform in vitro cell culture assays, qRT-PCR, molecular biology cloning, CRISPR gene editing, biochemistry, and next generation sequencing assays including single cell genomics.
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Maintaining cell culture equipment including cleaning, CO2 gas supply and routine contamination checks. Maintenance, cryopreservation, and cataloging of various cell culture lines.
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They use a combination of approaches including protein biochemistry and in vitro reconstitution, single-molecule biophysics, proteomics and genomics, fungal genetics, evolutionary analysis, mammalian cell culture, and state-of-the-art live-cell imaging.
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The company provides unrivaled service and quality to scientists working in biopharmaceuticals, cell therapy, and regenerative medicine, assisted reproductive technology and cytogenetics, and industrial cell culture for the large-scale production of biotherapeutics and vaccines.
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Experience in cell based functional assays (flow cytometry, immune cells activation, co-culture, autoimmune models, killing assays, proliferation, apoptosis). PhD in molecular and cellular biology, immunology, cancer biology, biochemistry, genetics, or a related field with zero to three years of relevant experience.
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Responsibilities include cell culture and maintenance of human cell lines, basic molecular biology (PCR, cloning, running DNA and protein gels), flow cytometry, preparation of samples for deep sequencing, confocal microscopy, basic statistical calculations, and other duties as assigned.
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The applicant will perform experiments that include molecular cloning, protein purification, cell culture, Western blotting, quantitative RT-PCR, immunofluorescent staining, Fluorescence in situ hybridization, and other cell biological assays.
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Experience in ELISA assay development, cell culture, gene expression with RT-PCR and/or enzyme kinetics is desirable. Educational/Training Requirement: For Research Associate: BS/MS in biology, biochemistry and other related fields For Scientist: PhD in biology, biochemistry and other related Basic understanding and training in Biology, Biochemistry and any related field.
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Request biochemistry and molecular biology skills: Western blotting, ELISA, ICC, IF, RNA/DNA, PCR, RT-PCR, Chip, Luciferase report assay, and/or cell culture of neuron, astrocyte, microglia, and human iPSC, etc.
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Master’s degree in biology, biochemistry, bioengineering, or related discipline with at least 1+ years of cell therapy manufacturing and/or process development experience. OR Bachelor's degree in biology, biochemistry, bioengineering or related discipline with at least 2+ years of cell therapy manufacturing and/or process development experience.
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Full scope covered is therapeutic modalities of biologics and vaccines produced in mammalian cell culture and microbial systems like yeast, bacteria and baculoviruses. ABOUT YOU Experience: Broad and long-term experience in the biologics/ biochemistry/ pharmaceutical industry in R&D and/or industrial development, industrial operations and GMP considerations, global CMC projects.
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