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Broad and strong experience with relevant cellular and antibody-based immunological techniques to understand functional expression in vivo of monoclonal antibodies through nucleic acid delivery (pharmacokinetic ELISA in animal sera, cell based cytotoxic neutralizing assay and bactericidal assays such as serum bactericidal or opsonophagocytic killing assays) is required.
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Performs purification and analysis of samples for upstream process development activities, including but not limit to POROS titer assay and spent media amino acid analysis. Mapp Biopharmaceutical, Inc. "Mapp" is a San Diego based, innovative biopharmaceutical company focused on addressing unmet medical needs through the development of life-saving monoclonal antibody treatment options to combat a variety of neglected and tropical infectious diseases, and in support of biodefense programs.
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Experience and interest in assay development (in vitro/ex vivo/in vivo) We combine this and other high-throughput protein engineering approaches with computational design to create antibody-like drugs and other biologics.
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Extensive working knowledge of biomarker and bioanalytical assay development and cutting-edge Omics methodologies in the clinic. Through this proprietary, versatile and modular approach, Entrada is advancing a robust development portfolio of RN, antibody- and enzyme-based programs for the potential treatment of neuromuscular, ocular, metabolic and immunological diseases, among others.
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Deep subject matter expertise in multiple key technical aspects of commercial immunoassay development; including but not limited to protein chemistry, antibody screening, antibody: bead conjugation, assay diluent formulation; multiplexing; preanalytical sample processing; scale-up, assay optimization and troubleshooting; multivariate data analysis; analytical validation; clinical validation.
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Ideal candidate will have a significant background in plate-based assay development and antibody characterization including HPLC and Octet. We require an Associate Scientist II who will continue the development and optimization of assay suites for our current 3 programs, and for upcoming research projects.
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Experience with primary neurons and iPSC-derived neurons, and their use in assay development, is beneficial. Strong understanding state-of-the-art methods and technologies used in the industry Tissue handling techniques for detection of gene and protein expression, including fixation, sectioning, IHC, histological staining, ISH MAbs/bi specifics - expertise in antibody protein structure & function, ability to apply protein engineering principles to mAb drug design.
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The Sr Scientist II, Analytical Development, Biologics CMC will support the CMC analytical development for multiple types of biologics including but not limited to antibody-drug conjugates (ADC), monoclonal antibodies and bispecifics.
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We are seeking an innovative and motivated Senior Scientist I to develop and implement novel capabilities and assays to functionally characterize our therapeutic antibody drug candidates and to help spearhead projects to continually improve and expand upon our internal capabilities.
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Mosaic teams provide comprehensive, end-to-end discovery services, including program assessment and strategy development; program management; antibody discovery and optimization; protein production, purification, and characterization; protein engineering; biochemical and cellular assay development; formulation, stability and developability studies; bioanalytical (PK and PD) assay development; and candidate-validating pharmacology.
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Biochemical techniques including quantitative Polymerase Chain Reaction (qPCR)/Digital Droplet PCR (ddPCR), Enzyme-Linked Immunosorbent Assay (ELISA), western blotting, gel-based assays, etc. The core platform technology comprises proprietary targeted lipid nanoparticles (tLNPs) that are composed of LNPs conjugated with a recombinant protein binder, such as a monoclonal antibody.
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Experience of managing technical aspects of assay development and implementation. Significant experience in the development of high throughput assays relevant to pre-clinical antibody drug discovery.
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We have the capabilities to analyze virtually any peptide, protein, or antibody, and have built a reputation for solving technical challenges related to assay development and validation.
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ResponsibilitiesExtract protein, DNA and/or RNA from necropsied animal tissueRun ddPCR, qPCR, ELISA, Western Blot etc analysis on tissue extractsCell culture, Neutralizing antibody assays and total antibody assaysSample analysis assay optimizationSOP writingData recording and analysisQC analysis This position directly impacts preclinical outcomes including proof of concept, ED50, MED, clinical hold response and IND enabling studies.
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Participates in projects for Biomarker/IVD assay development in various disease areas. The Research Associate I will support immunodiagnostic assay development following the product development process.
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